Journal: Oncology Letters
Article Title: Methylglyoxal-derived glycated albumin enhances the stemness potential of invasive ductal carcinoma-derived breast cancer stem-like cell line KAIMRC1
doi: 10.3892/ol.2026.15541
Figure Lengend Snippet: GA effects on ERK1/2 phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Article Snippet: Mouse primary monoclonal antibodies directed against extracellular signal-regulated kinase (ERK)1 (clone G-8; cat. no. sc-271269), phosphorylated-ERK1/2 (p-ERK1/2; clone E-4; Tyr204 of ERK1; cat. no. sc-7383), OCT3/4 (clone A-9; cat. no. sc-365509) and RAGE (clone E-1; cat. no. sc-74473) were obtained from Santa Cruz Biotechnology, Inc.
Techniques: Phospho-proteomics, Derivative Assay, Western Blot, Incubation, Expressing, Control